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The dried extracts were pretreated and loaded on Agilent 7890 gas chromatograph system (Agilent Technologies Inc., USA) coupled with a Pegasus HT time-of-flight mass spectrometer (LECO Corporation, USA) for GC-TOFMS analysis as described before 52

Evidence from the Institute for Social Marketing (University of Stirling), already shows that before, during, and after the implementation of standardised tobacco and the TPD, tobacco companies engaged in activities which may have disrupted and confounded the impact of the legislation on smoking attitudes and behaviour [4]

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Exact ratios are not officially specified and may vary slightly by production batch and regional regulatory formulation