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For measurements of BK channel function, the basolateral membrane of HBEC mounted in Ussing chambers was permeabilized with 20 M Amphotericin B (#A2411

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Luckily, there havent been many negative experiences with this, but there was this one time where we were playing and someone in the audience went to the box office and demanded their money back, saying so it wasn't a girl and stuff like that