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Bruno was the most popular pipe tobacco for many decades , particularly in the UK

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For every treatment, 10 seeds per Petri plate were taken

Study site and school selection Upper Egypt was selected due to its high rates of adolescent and adult smoking, early smoking initiation and socioeconomic challenges, especially in Asyut, the Egyptian governorate with the highest poverty rate and a demographic profile similar to national averages (15, 53, 54)