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Toxic emissions from all smoked cigarettes annually include an estimated 30006000 metric tons of formaldehyde and 12 00047 000 tons of nicotine (Novotny et al

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[29] Nicotine may result in arousal and wakefulness, mainly via incitement in the basal forebrain

National Toxicology Program released a Strategic Roadmap advising regulatory agencies to provide more human-relevant toxicology data while reducing the use of animals. Currently, the CDC has numerous options for studying vaping without animals, including in vitro assays, in silico approaches, computational chemistry and a range of sophisticated tissue models that include 3D organoids and organs-on-chips