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The cells were grown in Endothelial Cell Medium (ECM, ScienCell Research Laboratories, Carlsbad, CA, USA) supplemented with 10% FBS, 1% (v/v) penicillin/streptomycin, and 1% endothelial cell growth factors at 37 C with 5% CO 2 and 95% air

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Make sure to repaint the ceiling, too, since it will have had the most exposure to smoke

Walter H
