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Wieczorek J, Kaczor M, Boryo A (2022) Determination of 210Po and 210Pb in cannabis (Cannabis sativa L.) plants and products

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After systematic optimization of the entire workflow, where we varied circularized gDNA exonuclease treatments, base-editing reaction conditions and end-repair protocols, we identified an optimized protocol (Supplementary Note 1 and Supplementary Protocol) that consistently detected all 54 previously validated ABE8e-NRCH: HBB S -gRNA off-target sites, which we called CHANGE-seq-BE (Extended Data Fig