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After washing cells thrice with PBS, for permeabilization, the cells were treated with 0.2% Triton X-100 at RT for 10 min

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Like other miRNAs, miR34c can inhibit the transcription or initiate the degradation of target gene mRNAs by binding to the 3UTR (89)

United States farmers and ranchers provide us with more than $5 billion worth of agricultural products each year
