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Diagnosis primarily relies on clinical examination, but radiographs or biopsy should be employed when the origin of the pigmentation is unclear, to distinguish them from more serious conditions

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bassiana virulence against Helicoverpa armigera and Spodoptera frugiperda achieved by introducing a gene encoding the VRF1 protein, which is a component of the venom of the parasitoid wasp Microplitis mediator , into the genome of the fungus

According to World Health Organization (WHO), annually there are about 3-4 million new cases of infection[1,2]