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Briefly, for RP-HPLC analysis, an UltiMate 3000 HPLC from Thermofisher with a Fortis Bio C18, 3 m, 4.6 100 mm reversed-phase column was used and analysed using the Chromeleon software (v6.8)
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After staining, cells were fixed in 4% PFA, dried onto glass slides and examined by epifluorescence microscopy (Olympus, BX60)
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