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The assay mixture (1 mL) consisted of 2.75 mM L-tyrosine or L-DOPA in sodium acetate buffer (0.2 M, pH 5.0), 0.2 mM PLP, and an appropriate amount of enzyme in buffer (25 mM Tris-HCl and 150 mM NaCl, pH 7.4)
Essential compliance elements include: Proper labeling of every vial with concentration, lot, prep date, and operator
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