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The double increase in Asc in ischemic tissue compared to healthy tissue indicates increased ROS formation under ischemic conditions and confirms the role of oxidative stress in ischemia

Schenk BE 1 , Festen HP, Kuipers EJ, Klinkenberg-Knol EC, Meuwissen SG

However, the presence of internal thrombin cleavage sites in expressed proteins can severely affect the purification of intact proteins