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All cells except HepG2 were maintained in RPMI 1640 media (Gibco) containing 2mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin

Therefore, any disturbance in the dynamics of iron import, sequestration, or export can destabilize cellular iron homeostasis, impacting the propensity toward ferroptosis

Oxidative damage extends beyond the acute phase, disrupting the BBB, impairing vascular integrity, and activating inflammatory cascades that perpetuate secondary injury

Many people inject their stomach or thigh