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doi: 10.1016/j.bbi.2013.07.007 96 LlorensF.ThneK.TahirW.KanataE.Diaz-LucenaD.XanthopoulosK.et al
In brief, blood was drawn by median cubital vein venipuncture from healthy volunteers and centrifuged through a Ficoll Histopaque 1077 gradient (Sigma) to isolate mononuclear cells
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They proposed that increase in NO bioavailability would increase cardiac output and thereby affect cardiovascular hemodynamics
