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These are available at The following three imageJ macro scripts are required: 00-Preporcessing 01-Anisotropy Macro 03-ROI Selection The images were background-corrected using a rolling ball filter where the radius is larger than the largest cell in your images but small enough that distortions in the background are removed, in this case a radius of 100 was used

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The major pathological hallmarks of PD are represented by the progressive loss of dopamine(DA)-ergic neurons in the substantia nigra (SN) of the central nervous system (CNS) associated with cytoplasmic inclusions termed as Lewy bodies and Lewy neurites (Spillantini et al., 1997)