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Long-term regulation of cellular fatty acid uptake occurs via changes in CD36 gene transcription, mediated, among others, by fatty acid-induced PPAR activation, HIF-1, and C/EBP ( In addition, CD36 is heavily N-linked glycosylated ( A small portion of UDP-GlcNAc acts as a versatile substrate pool for reversible post-translational modifications known as O-GlcNAc

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The mixture was incubated at room temperature in the dark for 20 min, then immediately placed on ice for cooling