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Blots were developed by chemiluminescence method using Amersham ECL+ chemiluminescence kit

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Leveraging this strategy, the same group designed a method to generate a C-terminal ubiquitin (Ub) aldehyde from Thz-caged Ub using a Pd-mediated cleavage reaction, resulting in the inhibition of cellular deubiquitinases (DUBs) that exclusively cleave the isopeptide bond between Ub and a protein substrate 72

[DOI] [PMC free article] [PubMed] [Google Scholar] 87.Zhang D, Ruan J, Peng S, Li J, Hu X, Zhang Y, Zhang T, Ge Y, Zhu Z, Xiao X, Zhu Y, Li X, Li T, Zhou L, Gao Q, Zheng G, Zhao B, Li X, Zhu Y, Wu J, Li W, Zhao J, Ge W, Xu T, Jia J-M