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Four hours later, cells were washed with PBS and detached with TrypLE (Gibco)

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[97] demonstrated that in spontaneously hypertensive rats with a high risk of stroke, supplementation with AST or vitamin E reduced systolic blood pressure, delayed cerebral vascular thrombosis, increased the nitric oxide (NO) metabolic rate, elevated urinary NO/NO levels, and significantly decreased 8-OHdG levels