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Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)

The study was conducted with fifteen women with an average age of 42 who applied the nanoemulsion twice a day for four weeks

This is especially true of therapeutic areas in which little or nothing is known about the probable PK/PD relationship and where the intended indication is a disease with dire consequences, such as cancer