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These results indicated that folate supplementation during pregnancy could regulate the gut microbiota of male offspring and improve gut barrier integrity, and folate inhibited the expression of the TLR4/NFB pathway in the liver, thereby ameliorating lipid metabolic disorders and alleviating hepatic steatosis

The biotin switch assay has also been used

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Fixed ammonia is subsequently assimilated into glutamate through the glutamine synthetase/glutamate:2-oxoglutarate aminotransferase (GS-GOGAT) pathway, and the glutamate thus formed is then used for cell growth and the renewal of nitrogenase to maintain diazotrophy 11,12