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Description
Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)
Beads were washed once with lysis buffer (without benzonase) and 4 times in TBS
These inhibitors differ in their selectivity, potency, onset and duration of action, cost, administration considerations, precautions, and adverse effects profiles
Transplantation (2013) 95:9605
