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Monocyte isolation and cell culture Human peripheral blood mononuclear cells (PBMCs) were isolated from the obtained leukopaks using density gradient centrifugation with Ficoll-Paque Plus (Cytiva, Marlborough, MA)

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When adverse effects occurred, they were typically mild and transient, with the most common being mild to moderate headaches (similar frequency to placebo groups), minor injection site reactions (redness, slight swelling), and gastrointestinal effects at higher oral doses (nausea, diarrhea)