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This is not a theoretical concern it is one of the most common sources of non-reproducible cell biology data (Munford, 2008)

This prevents a vacuum from forming and makes it significantly easier to pull the liquid into the syringe smoothly and accurately
[DOI] [PMC free article] [PubMed] [Google Scholar] 138.Setin-Suero E., Surez-Pinilla P., Ferro A., Tabars-Seisdedos R., Crespo-Facorro B., Ayesa-Arriola R

It depends on the patient
