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doi: 10.1126/science.164.3878.435
Because mitochondrial -oxidation facilitates the degradation of stored fats for the production of energy, we suspected that nhr-49(nr2041) worms might be high in fat due to the reduced expression of these key -oxidation enzymes
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At an OD 600 of 0.60.7, expression was induced with 1 mM IPTG and the culture was continued up to an OD 600 of 3.0 where the cells were harvested at the end of the exponential growth phase by centrifugation (10 min, 3,220 g , 4C)
