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Requires 200 1000 g lysate starting material per assay The Rho-GTP binding domain (RBD) of the human Rhotekin protein has been overexpressed as a GST-tagged recombinant protein in a bacterial expression system and bound to colored glutathione Sepharose beads

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Cell Sci

R., Andrikopoulos, S., van der Poel, C., and Stupka, N
