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After boiling, 900 L of ice-cold IP Lysis buffer was then added to the sample, and cells were left on ice for 20 minutes with vortexing every 5 minutes for 10 second

FIGURE 3 Furthermore, MET levels in post-mortem temporal lobe samples of autistic or healthy individuals were analyzed in other studies, showing a reduction in MET transcripts and proteins in patients with ASD compared to control ones (Sugihara et al., 2007) and children with co-occurrence of gastro-intestinal diseases

Quantitative real-time PCR was performed using SYBR Green Gene Expression Assays

Additionally, seven studies are currently recruiting participants