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Introduction It has been 15 years since the discovery of ghrelin, a peptide hormone related to, among others, energy balance, feeding, stress, anxiety, and reward

GHK-Cu is supplied as a lyophilised powder and must be reconstituted with bacteriostatic water before use in any laboratory protocol

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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain