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SiRNA transient transfection was performed twice on consecutive days using 40 nM, non-targeting siRNA (si CTRL), siRNA against human ATG5 (si ATG5), ULK1 (si ULK1), ATG7 (si ATG7), and DRP1 (si DRP1) purchased from Dharmacon (D-001810, L-004374, L-005049, L-020112, and L-012092 respectively)

doi: 10.1111/andr.12198

Based on these results, several clinical trials have evaluated the potential use of EVs as biomarkers for the prediction of MDR development in patients with breast cancer, PC, colon cancer, pancreatic cancer, malignant melanoma, lung cancer, and multiple myeloma, which have been extensively reviewed elsewhere (317)

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