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Proteins were resolved by SDS-PAGE using NuPAGE 412% Bis-Tris precast gels (Fisher Scientific, WG1402BOX) and then transferred to 0.45 M nitrocellulose membranes (VWR, 10120-006)

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Once ingested, pyridoxine is converted into its active form, pyridoxal phosphate (PLP), which plays a pivotal role in various metabolic processes, including: Amino Acid Metabolism: PLP actively participates in the metabolism of amino acids the building blocks of proteins

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