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Library preparation and RNA sequencing (RNA-Seq) For genome-wide analysis of gene expression, RNA sequencing libraries from isolated mRNA were generated and sequenced by the Institute for Lung Health (ILH) Genomics and Bioinformatics at the Justus-Liebig-University (JLU) Giessen (Germany)

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GST tagged fusion proteins bind to glutathione agarose (column 2) and, after washing, are eluted with elution buffer with excess glutathione (column 3)

The data unveiled that the combination of sulforaphane and fluorouracil is more effective in enhancing the process of apoptosis than fluorouracil alone