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Coli containing expressed target fusion protein PBS/EDTA: 1X PBS, 5 mM EDTA, pH 7.4 Lysis buffer: 50 mM Tris, 50 mM NaCl, 5 mM EDTA, 1 g/ml leupeptin, 1 g/ml pepstatin, 0.15 mM PMSF, 1 mM DFP, 1 mM 2-ME, pH 8.0

Consistently with this kinetic, the presence of serum anti-nuclear antibodiesevidence for adaptive immune responsesdetected at first evaluation of Raynauds is considered an important, independent predictive element to classify Raynaud as secondary to SSc [68, 69]

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BBB penetration is facilitated by the compound's lipophilicity and small size, though the exact mechanism of CNS entry (passive diffusion vs