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ROS stimulates the activity of many physiological components, including mitogen-activated protein kinases (MAPK), tyrosine kinases, Rho kinases, and transcription factors (NF-kB, AP-1, and HIF-1) and increased concentration of intracellular free Ca 2+ also inactivates the protein tyrosine phosphatases (PTP)

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We also assessed the neuronal pathological changes in acquired digital images saved as uncompressed 24-bit RGB TIFF files in the software program AnalySIS (Olympus Soft Imaging System GmbH, Munster, Germany), performing quantitative analysis of neuronal damage in the karyopyknotic areas