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Nat Rev Mol Cell Biol 25(1):1333

So without incorporating new principles into a new lifestyle, people simply gain the weight back, and yo-yo dieting is bad for the body and overall health as well as being demoralizing

If you're going to use the entire vial within 4-6 weeks, keeping it in the refrigerator is actually safer and more effective

Glass tubes are then placed at 20 C on a gentle tube shaker for 8 h
