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Detection of cellular ROS, apoptosis, DSB, viability and colony formation capability LLC cells were seeded in a 24-well plate at a density of 10 5 cells, cultured at 20% O 2 for cell adherence, and further incubated at 1% O 2 for 24 h to induce cells to become hypoxic

Cells were centrifuged and resuspended in 0.1 M sodium cacodylate buffer and post-fixed for 1 h with 1% osmium + 0.1 M sodium cacodylate buffer, then dehydrated and embedded in Agar 100 resin

Cell (2016) 165:27687

Biochem Pharmacol 65:857875 Hunt MC, Alexson SE (2002) The role Acyl-CoA thioesterases play in mediating intracellular lipid metabolism