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After washing three times with PBS, the liver tissues were homogenized by adding 0.1 M Tris-HCl buffer (pH 7.4) in a volume 10 times the tissue weight

doi: 10.1371/journal.pgen.1007392

Fluoxetine interacts with the lipid bilayer of the inner membrane in isolated rat brain mitochondria, inhibiting electron transport and F1F0-ATPase activity

These molecules oxidize a variety of cellular constituents, but sulfur-containing amino acid residues are especially susceptible [28]
