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His-561 is equivalent to His-262 in IdeS and gasMac-1, where the N 2 atom of the side chain forms a hydrogen bond with the S atom of Ser/Cys-94

doi: 10.3389/fchem.2023.1249472 Received 28 June 2023 Accepted 04 September 2023 Published 15 September 2023 Volume 11 - 2023 Edited by Graeme Stasiuk, Kings College London, United Kingdom Reviewed by Lakshmi Narayanan Mosur Saravana Murthy, Intel, United States Manuel Baobre-Lpez, International Iberian Nanotechnology Laboratory (INL), Portugal Updates Copyright 2023 Liu, Guo, Li, Jia, Xie, Wang, Tian, Li and Hao

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The most common cause of methaemoglobinaemia is exposure to an oxidising agent, such as in nitrite poisoning, however it can also be caused by a variety of genetic defects affecting both red cell metabolism and Hb structure 3