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In the present study, we used young adult mice to investigate epigenetic responses, because, by this age, PA markers 26 stabilise at an elevated level, and epigenetic changes are expected, but without the confounding effects of oxidative damage that emerge in later life 27

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The use of an in-vitro batch fermentation (human colon) model for investigating mechanisms of TMA production from choline, L-carnitine and related precursors by the human gut microbiota

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