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Choline helps the liver process and excrete chemical waste products within the body, and its also required for the transport and metabolism of fats and cholesterol, which is vital for the healthy functioning of the liver

SP can target the macrophages to accelerate the protein degradation of Kelch-like ECH-associated protein 1 (KEAP1) via microtubule-associated protein 1 light chain 3 (LC3)-mediated autophagy and ubiquitin-mediated proteasome degradation

After the mixture cooled down, the absorbance value at 500 nm was measured using a microplate reader

Global DNA methylation (5-mC) level Levels of 5-methyl-2-deoxycytidine (5-mC) were assessed in liver tissue and cultured cells using the 5-mC DNA ELISA Kit (Zymo Research, Irvine, CA)