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This starts with in vitro studies on various cell lines to determine cytotoxicity, followed by in vivo studies using animal models to evaluate systemic toxicity and establish safe dosage levels [39,49,56]

Carnitine has been shown to be an endogenous substance with anti-inflammatory properties [47], as it inhibits the activity of NF-B and other core transcription factors involved in the inflammatory response and effectively reduces the levels of proinflammatory cytokines (e.g., IL-1, IL-6, and TNF-) [48, 49]

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Amreddy N, Muralidharan R, Babu A, Mehta M, Johnson EV, Zhao YD, Munshi A, Ramesh R (2015) Tumor-targeted and pH-controlled delivery of doxorubicin using gold nanorods for lung cancer therapy