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To stop enzymatic activity, the cell suspension was washed again with cold DMEM/F12 and centrifuged (5 min, 4 C, 300 g )
Monitor progress over 4-6 weeks for noticeable changes
Arbutin is a safer and gentler alternative to hydroquinone, but its effectiveness compared to hydroquinone is still unknown
N-acetylcysteine is a chemical reducer of disulfide bonds via its free sulfhydryl groups may interact with the extracellular disulfide bridges of angiotensin II receptor, alter its tertiary structure, and inhibit the binding of angiotensin II to its surface receptors (AT 1a receptors) with subsequent attenuation of signal transduction and cell action
