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Gourds or cucurbits are typically climbers or trailers and include significant economic plants such as watermelons, cucumbers, squash, and luffas 3

Rigorous validation of metabolic markers (e.g., plasma PKM2, specific lipid species, and mitochondrial DNA) is essential for early diagnosis, disease activity monitoring, and treatment response prediction

CCK8 assay For cytotoxicity assay, the cells grown in the logarithmic phase were seeded into 96-well plates (7 10 3 cells/well) for 24 h, then exposed to different concentrations (10, 5, 1, 0.1, 0.01 and 0.001 M) of the test compounds for 72 h

From the age of 30-35