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In conclusion, the findings of this study indicate that all examined biomarkersinterleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor-alpha (TNF-)may serve as valuable prognostic indicators for potential complications associated with diabetes

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Blood was collected in red-top tubes, centrifuged at 500 g for 10 min and serum was aliquoted into cryopreservation tubes and stored at 80 C until shipped to Metabolon for metabolomics analysis

Finally, the cell nuclei were counterstained (hematoxylin, 10 min, 25C
