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This is related to copper sensitivity in some patients

Protein extracts from both WT and GR mutant strains were obtained through ultrasonication, separated by 10% SDS-PAGE, and electroblotted onto membranes at 100 V for 90 min

The glyoxylate shunt reduces endogenous ROS production, while the pentose phosphate pathway is enhanced to increase NADH levels and replenish antioxidants