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After phase separation, the upper phase was collected and vacuum-dried

CB1 Cannabinoid Receptors Promote Oxidative Stress and Cell Death in Murine Models of Doxorubicin-Induced Cardiomyopathy and in Human Cardiomyocytes

After incubation, the cells were harvested by trypsinization

These studies demonstrated a rather unique and unconventional pattern of 3 -AR desensitization that may be animal and cell specific (Nantel et al., 1995