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Description
Culture and differentiation of primary human bronchial epithelial cells (phBEC) Normal primary human bronchial epithelial cells (phBEC) from three healthy donors were obtained from Lonza (Wokingham, UK), cultured in BEGM medium (Lonza) and differentiated at the air-liquid interface (ALI) in PneumaCult-ALI medium (Stemcell Technologies, Cologne, Germany) for up to 2833 days as described previously 67
Metastasis 22 , 523532 (2006)
Methods 25: 402408
Rapidly Restores Hydration Dehydration thickens mucus, worsens fatigue, and slows immune responses
