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Protein adsorption experiments 1 mg mL 1 of LAAM GSH-CQDs, commercial Lipo 2000 reagent (Sigma-Aldrich), and GO-CQDs prepared from heating the citric acid powder were mixed with 20 times diluted FBS in a volume ratio of 1:1, followed by vortexing for 30 s and incubation at 37 C for 30 min

Given the extensive role of HIF-1 in GBM progression, metabolic adaptation, and therapy resistance, it remains a critical target for novel therapeutic interventions aimed at disrupting tumor survival mechanisms in hypoxic microenvironments

Then, the changes in O 2 concentration were determined in 2 min by a dissolved oxygen analyzer