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A similar version of this genetic modification was reported also by another lab [64] , and both papers reported that chronically high levels of circulating GDF11 were surprisingly well tolerated in mice
Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development
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PMID 15256721
