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Apoptosis was determined by measuring internucleosomal DNA fragmentation by using a cell death detection ELISA kit (Cell Death Detection ELISA, Roche Molecular Biochemicals, Indianapolis, IN) according to the instructions provided by the manufacturer

The OCR and extracellular acidification rate were measured using a Seahorse XFe24 analyzer (Agilent), with addition of oligomycin (1 M), FCCP (1 M), and antimycin A and rotenone (0.5 M each)

Coenzyme Q10 ameliorates the quality of mouse oocytes during in vitro culture

These defects may persist in offspring, predisposing them to metabolic and neurodevelopmental disorders, underscoring the intergenerational consequences of sperm redox imbalance