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On March 3, the researchers published a letter in the New England Journal of Medicine describing a series of 12 delayed injection site reactions including swelling and rashes that they say require further investigation

Immunofluorescence Fibroblast cultures at passage 3 were cultured in eight-well chamber slides (Gibco, Burlington, ON, Canada) in growth medium (DMEM, 10% fetal bovine serum (FBS), penicillin, and streptavidin from Invitrogen, Burlington, ON, Canada)

Free radicals such as ROS and NO, as well as cytokines in the inflammatory microenvironment, can interfere with the normal function of the ER, promoting abnormal protein folding within the ER and exacerbating ERS (Hasnain et al., 2012)