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For the detection of transgenes, DNA was subjected to qPCR, and SYBR Premix ExTaqII (Takara Bio, Shiga, Japan) was used for the detection
specifically its potential to accelerate healing of tendons, ligaments, muscles, and bones
doi: 10.1038/nature12517
Cultured hepatocytes exhibit basal GCLC phosphorylation, which increased when treated with DBcAMP or phenylephrine, suggesting GCLC may be under a basal inhibitory tone [128]
