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A: In vitro studies involve cells in a controlled environment, using molar concentrations (M), while in vivo studies involve whole organisms and use weight-based dosages (mg/kg)

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In addition, impaired function of vascular endothelial cells can lead to increased vascular permeability and abnormal vasoconstriction function, affecting the blood supply to the brain and aggravating nerve damage (Shi et al., 2025)